Detection of Renibacterium Salmoninarum in Tissue of Brook Trout (salvelinus Fontinalis) by Nested Rt–pcr
نویسنده
چکیده
Pathogenic bacterium Renibacterium salmoninarum causes kidney disease with high mortality rate and considerable economic losses in salmonid farming. Thus, application of fast and sensitive method for R. salmoninarum diagnosis is of great importance. This paper describes detection of R. salmoninarum in brook trout tissue with gross clinical signs of disease by nested RT–PCR. Determination of partial sequence of bacterial msa gene was done prior to comparison with similar sequences from different R. salmoninarum isolates. Nested RT–PCR proved to be a rapid and valuable diagnostic tool for R. salmoninarum detection, and sequence analysis confirmed previously reported genetic uniformity of this bacteria.
منابع مشابه
Evaluation of otolith shape variability in hatchery-reared brook trout (Salvelinus fontinalis), Black Sea trout (Salmo trutta labrax) and their hybrid
In aquaculture, the process of hybridization is used to produce animals that have better growth rates, meat quality, and higher resistance and tolerance to environmental variations than their parental species. The identification of a hybrid fish may require a DNA-based technology that is an expensive process. In this study, the sagittal otolith shape of hatchery-reared brook trout (Salvelinus f...
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A nested reverse transcriptase (RT) PCR assay detected mRNA of the salmonid pathogen Renibacterium salmoninarum in samples of RNA extracts of between 1 and 10 cells. Total RNA was extracted from cultured bacteria, Atlantic salmon (Salmo salar L.) kidney tissue and ovarian fluid seeded with the pathogen, and kidney tissue from both experimentally challenged and commercially raised fish. Followin...
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Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.
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